We showcased WebAtlas on a 'triple' modality atlas of the developing human hindlimb from Teichlab that combines scRNAseq, Visium spatial RNA-Seq and In Situ Sequencing (ISS done by Kenny Roberts in my lab). You can explore it on tinyurl.com/4behu43y & few more tweets below.

'famous nephrologist' Sandeep K. Mallipattu, scrnaseq expert Rob Bronstein, bridge award winner Nina Cintron Pregosin, ivy league Jesse Pace, #podo23

Then we checked it’s clustering performance also in an ancillary PBMC multiome dataset having both scRNASeq & scATACSeq from same cells. It seeming performing well there as well.

9/

I am recruiting! I am looking for a PostDoc to study #maize leaf development using scRNAseq Institute of Molecular Plant Sciences #PlantSciJobs #genetics Apply here:elxw.fa.em3.oraclecloud.com/hcmUI/Candidat… Reach out via email if you are interested and want to know more!

I'm really excited to be going to the CAN ACN meeting in Montreal this week! Our lab has 5 posters, spanning scRNAseq, splicing, ephys, and even mouse behavior (courtesy of Shernaz Bamji's lab). Also, tomorrow is my bday! #canacn2023

New @bioconductor package from our lab demuxSNP.

#scRNASeq studies using HTO multiplexing to load >1 sample per lane. The cells tags should assign cells to samples but it’s problematic.

demuxSNP can QC & increase assignment of cells to samples.

bioconductor.org/packages/devel…

Dr. Marcia R. Cruz-Correa: A Focus on Cancer Health Disparities

In our new blog post, we'll take you through some of the key publicly available scRNA-seq data repositories, and show you how to start searching for #scRNAseq public data!

Read here: bit.ly/41ZQHmT

#singlecell #publicdata

For those attending the #NeuroFrance2023 meeting organized by SociétéNeurosciences, come and see the posters from our lab presented by Léa, Antoine de Chevigny and Aurélien!

Cortical development, neuronal identity, circuit formation, scRNAseq and other fancy methodological approaches!

Figure 1: Integrated analysis of all 13 participant samples.
scRNAseq reveals multiple immune & kidney cell populations, with immune cell populations dominated by rejection samples.

In BELAs, but not in VE cysts, we found a small cell population that expressed AVE markers, and that mapped to AVE cells annotated in recent scRNAseq datasets from the mouse embryo. AVE cells were found in small patches on many BELAs.

Excellent meeting with Patrick Lacasse from 10x Genomics today. #scRNAseq #snRNAseq #multiplexing #singlecells #transcriptomics
Single Cell Precision Inc

vs 免疫療法の副作用

チェックポイント阻害剤による治療中の自己免疫毒性の原因を調べるべく、scRNAseqを実施。で、有害事象が起きてる人では、CXCR6+ IFN-γ 細胞傷害性CD8+ Tがクローン的に増加することが明らかに。また、CD4+ TからのIL-21がこれらを駆動することも特定

ナイスな戦略につながりそ!

Table 2. Participant information for temporal scRNAseq.
Treatment & rejection information for participants with longitudinal samples to assess response to anti-rejection therapies.

✔️Integration of ncRNAseq with scRNAseq identifies aberrant population of distinct “alveolar intermediate cells”

✔️These cells are also found in early PF biopsies

✔️And of course brings it back to the patient with a novel concept to CurePF called the PF Connectome⚡️

#ATS2023

免疫学研究者からscRNAseqの解析方法について聞かれる事が最近多いので再掲↓

様々な解析方法が説明されてます。

bigbioinformatics.org/intro-to-scrna…

Long read scRNAseq 😂

Ecstatic to share that our recent work using scRNAseq to study kidney transplant rejection has been published in Journal of Clinical Investigation!
jci.org/articles/view/…
Hildeman Lab David Hildeman E Steve Woodle MD CCHMC Immunology Grad Program University of Cincinnati Office of Research The Graduate College at University of Cincinnati

Summary 🧵 below:

That moment when you finally jumped through the last hoop to submit your scRNAseq data to dbGaP!

'My MD thinks I'm top bucket material (I'm actually clueless). It's because I read Finance Homie (and he has no idea it exists).'

Deep Background on the DC Elite
matt-wolfson.com/news/tony-blin…

MCBI (Amsterdam UMC locatie VUmc) Cancer Center Amsterdam - Amsterdam UMC Amsterdam institute for Infection and Immunity Ernesto Rodríguez Lotte de Winde Febe van Maldegem Amsterdam UMC Our final line up of speakers before the summer break🌞😎: Today, Tara Muijlwijk will share their #flowcytometry & #scRNAseq analyses of #headneckcancer TME. In just 2 weeks, Madelon de Jong will present how #scRNAseq delineates neutrophil-stromal cell axis in #MultipleMyeloma !

Had the opportunity to present our work on the integration of scRNAseq and ST at the yearly 10x Genomics User Group in Madrid -- Great experience and opportunity to connect with the Spanish single-cell community! 🧑‍💻🧬🧑‍🔬